Histochemistry of Oral Tissues

Which stain is commonly used to detect collagen fibers in oral tissues?

• Periodic acid–Schiff (PAS)

• Masson’s trichrome

• Alcian blue

• Sudan black

What does the PAS stain primarily detect?

• Lipids

• Proteins

• Carbohydrates and mucopolysaccharides

• Collagen

Which stain is best for demonstrating elastic fibers?

• Van Gieson

• Verhoeff’s stain

• PAS

• Hematoxylin and eosin

Which of the following stains is used to identify glycogen in oral tissues?

• Alcian blue

• PAS

• Masson’s trichrome

• Toluidine blue

What is the primary function of Hematoxylin in H&E staining?

• Stains cytoplasm pink

• Stains nuclei blue or purple

• Detects lipids

• Stains collagen fibers green

Which of the following best describes the principle of histochemical staining?

• Mechanical cutting of tissues

• Use of enzymes for digestion

• Chemical affinity between tissue elements and dyes

• Antibody-antigen reaction

Which stain combination is routinely used in diagnostic histopathology?

• PAS and Sudan black

• H&E (Hematoxylin and Eosin)

• Trichrome and Silver stain

• Alcian blue and Toluidine blue

In H&E staining, eosin binds to which cellular structures?

• Nucleic acids

• Cytoplasmic proteins

• DNA only

• Lipids

What is the typical color of cytoplasm in H&E-stained tissue sections?

• Blue

• Purple

• Pink

• Green

Which dye in H&E staining colors the nucleus?

• Eosin

• Hematoxylin

• Toluidine blue

• Alcian blue