Chromatography and Electrophoresis Quiz

The principle of chromatography is based on:

• Density differences

• Differential solubility

• Differential migration of solutes between phases

• Evaporation

In paper chromatography, the stationary phase is:

• Solvent

• Water

• Paper

• Silica gel

In Thin Layer Chromatography (TLC), the stationary phase is typically:

• Paper

• Cellulose

• Silica gel or alumina

• Polyacrylamide

The Rf value in chromatography is defined as:

• Distance traveled by solvent ÷ sample

• Distance traveled by sample ÷ solvent

• Distance from origin ÷ solvent front

• Sample thickness ÷ solvent polarity

Which type of chromatography uses charged resins to separate molecules?

• Affinity chromatography

• TLC

• Ion-exchange chromatography

• Gas-liquid chromatography

In ion-exchange chromatography, cation exchangers bind:

• Negatively charged molecules

• Positively charged molecules

• Neutral molecules

• Only proteins

Affinity chromatography is based on:

• Size exclusion

• Specific binding interactions

• pH gradient

• Solubility

In affinity chromatography, ligands are attached to:

• Sample

• Elution buffer

• Solid support matrix

• Mobile phase

Gas Liquid Chromatography (GLC) uses which mobile phase?

• Liquid

• Solid

• Gas

• Gel

In GLC, the stationary phase is:

• Gas

• Liquid coated on a solid support

• Inert gas

• Silica gel