Electrophoresis Lab PreQuiz

What is the first step in casting a gel using the MiniOne® Casting System?

• Pour the agarose solution into the gel tray.

• Place the casting stand on a level surface and insert the comb.

• Microwave the GreenGel™ cup.

• Remove the gel tray with solidified gel.

What is the maximum number of gel cups you should microwave at a time?

• 2

• 3

• 4

• 5

What is the volume of each sample loaded into the lanes?

• 5 µL

• 10 µL

• 15 µL

• 20 µL

What should you avoid doing until the gel solidifies?

• Adding more agarose

• Disturbing the gel

• Removing the comb

• Placing the gel tray in the stand

Where should the power supply be plugged into the MiniOne® Carriage?

• Into the front of the carriage.

• Into the side of the carriage.

• Into the back of the carriage.

• Into the top of the carriage.

What should be done to help visualize the wells when loading?

• Turn on the high intensity white light.

• Turn on the low intensity blue light.

• Use a magnifying glass.

• Use a flashlight.

What should you do once the gel is loaded?

• Move it to a different location.

• Make sure the power supply is plugged in and place the photo hood on the carriage.

• Turn off the power supply.

• Remove the gel from the carriage.

What will prevent the green power LED from turning on?

• The tank is properly placed inside the carriage.

• There is enough buffer in the tank.

• The power supply is not plugged in.

• The photo hood is on the carriage.

How often should students check the migration of the bands?

• Every 10 minutes.

• Every 15 minutes.

• Every 5 minutes.

• Every 20 minutes.

What effect does light have on the fluorescent DNA signal during the run?

• It strengthens the signal.

• It has no effect.

• It weakens the signal.

• It changes the color of the signal.

Tags

NGSS.HS-PS4-3

NGSS.HS-PS4-5