Biotech webXam Review Strand 3.6 Part 2

What is the first step in cloning vector preparation?

• Transformation of host cells

• Isolation of the vector DNA

• Selection of transformed cells

• Gene insertion into the vector

Which of the following is a common method used to introduce exogenous DNA into bacteria?

• Electroporation

• Centrifugation

• Filtration

• Precipitation

What is the purpose of using antibiotic resistance genes in cloning vectors?

• To enhance bacterial growth

• To select for transformed cells

• To increase DNA replication

• To inhibit bacterial metabolism

Which of the following is NOT a method of bacterial transformation?

• Heat shock

• Electroporation

• Conjugation

• Filtration

What is the role of a plasmid in genetic engineering?

• To act as a host cell

• To serve as a vector for gene insertion

• To degrade unwanted DNA

• To replicate independently of the host genome

Which of the following is a benefit of genetically modified plants?

• Increased susceptibility to pests

• Reduced nutritional value

• Enhanced resistance to diseases

• Decreased growth rate

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NGSS.HS-LS2-7

What is the main goal of genome sequencing?

• To create new species

• To determine the order of nucleotides in DNA

• To increase genetic diversity

• To eliminate genetic mutations

Which molecule is NOT considered a large biological molecule?

• Carbohydrates

• Lipids

• Proteins

• Water

What is artificial selection primarily used for in agriculture?

• To increase genetic mutations

• To breed plants and animals with desirable traits

• To decrease crop yield

• To eliminate all genetic variation

Which of the following best describes a genetically modified organism (GMO)?

• An organism with naturally occurring mutations

• An organism with altered DNA through genetic engineering

• An organism that has been selectively bred

• An organism that has not been altered genetically