Introduction to (bio) process design

Which of the following represents the key steps in process design methodology?

• Brainstorming, prototype testing, budgeting, final implementation

• Target & specification, data collection, generate design & redesign, assessment & comparative analysis, final design and selection

• Data collection, random selection, trial and error, process execution

• Specification review, immediate implementation, troubleshooting, final approva

For target and specification, a bioprocess must clearly define:

• Goal of the (bio)process (e.g., product, purity, etc)

• Specification of the bioprocess and product (e.g., battery limits, production scale, process constraints, etc)

• Key performance indicators (e.g., production cost, environmental impact reduction sdg, etc)

• All of the above

Typical data collection includes (bio)reaction conditions. Which of the following are examples of bioreactor condition data?

• Daily maintenance schedule, cleaning procedure, and equipment purchase date

• Bioreactor material type, operator name, and laboratory location

• Substrate concentration (e.g., glucose), temperature: 35°C, pH: 7.0, nutrients: NH₃/O₂

• Market demand trends, competitor analysis, and sales forecast

Typical data collection includes (bio)reaction performance. Which of the following are examples of bioreaction performance data?

• Concentration of product (e.g., PDO: 3 mol/kg), reaction rate: 0.0245 h⁻¹, yield: 0.325 g PDO/g glucose

• Bioreactor volume, agitation speed, and aeration rate

• Temperature, pH, and nutrient composition

• Operator experience, lab location, and equipment maintenance records

Which statement best describes the relationship between upstream, bioconversion, and downstream processes?

• Upstream focuses on product separation, bioconversion purifies the product, and downstream cultivates microorganisms.

• Upstream and bioconversion are independent processes with no impact on downstream processing

• Bioconversion occurs after downstream processing to ensure product purity.

• Upstream prepares raw materials and microorganisms, bioconversion produces the desired product, and downstream processes recover and purify the product.