SDS-PAGE

What is the primary purpose of SDS in SDS-PAGE?

• a) To stain proteins

• b) To give proteins a uniform negative charge

• c) To denature DNA

• d) To increase protein migration speed

What property of proteins does SDS-PAGE separate them by?

• a) Charge

• b) Shape

• c) Size (molecular weight)

• d) Hydrophobicity

What is the role of the stacking gel in SDS-PAGE?

• a) To slow down protein migration

• b) To concentrate and align protein samples before they enter the resolving gel

• c) To separate proteins based on charge

• d) To stain the proteins

Which dye is commonly used to visualize proteins after SDS-PAGE?

• a) Ethidium bromide

• b) Coomassie Brilliant Blue

• c) SYBR Green

• d) Bromophenol Blue

What is the typical charge of proteins in SDS-PAGE?

• a) Positive

• b) Neutral
  

• c) Negative

• d) Variable depending on the protein