RP Microbiology

Why is aseptic technique important in this experiment?

• It prevents the introduction of additional fungi and protists that could contaminate the experiment.

• It prevents the introduction of additional bacteria that could contaminate the experiment.

• It prevents the agar from setting properly.

• It increases the temperature for bacterial growth.

What is the purpose of sterilising the agar plates before use?

• To help the bacteria grow more effectively.

• To prevent contamination by any bacteria that might be present on the plates.

• To make the agar more solid.

• To encourage the growth of aerobic bacteria.

Why is the inoculating loop sterilised before use?

• To ensure it is clean.

• To prevent contamination from any bacteria that might be present on the loop.

• To heat the bacteria before applying it to the agar plate.

• To increase the efficiency of bacterial growth.

What is the purpose of incubating the agar plates at 25°C in schools?

• To encourage the growth of harmful bacteria.

• To ensure that the bacteria grow more quickly.

• To reduce the risk of growing harmful pathogens

• To prevent bacteria from growing at all.

What does a "zone of inhibition" indicate in the experiment?

• Where bacteria are growing.

• Where bacteria are spreading.

• Bacteria have been killed

• The antimicrobial wasn't applied.

Why should the Petri dish lid be secured but not fully sealed?

• To stop anaerobic growth.

• To let oxygen in while preventing contamination.

• To make the lid easier to remove.

• To prevent condensation.

What measures the effectiveness of antimicrobial solutions?

• The amount of bacteria.

• The area of the zone of inhibition.

• The size of the coloured area around each antimicrobial

• The colour of the agar.

What is the purpose of a control disk?

• for comparison

• for measurement

• to speed up bacterial growth

• to provide nutrients in the growth medium

If an antibiotic is ineffective, what will you see?

• Large zone of inhibition.

• Small or no zone of inhibition.

• Bacteria spreading rapidly.

• Antibiotic dissolving in the agar.

The formula for the area of a circle is πr2\pi r^2πr2. What is the area of a circle with a radius of 3 cm?

• 9 cm²

• 18 cm²

• 28.27 cm²

• 31.42 cm²