Quizs- ABE Lab- Micropipetting and Centrifuge Techniques

What would you set the volume window to if you were pipetting 6.5 uL using a p-20?

• 020

• 065

• 100

• 050

When uptaking the liquid into a micropipette, why must you slowly release the plunger?

• To ensure accurate pipetting and prevent splashing of liquid into the pipette.

• To increase the speed of liquid uptake.

• To avoid contamination of the liquid sample.

• To make the pipetting process more efficient.

What is the purpose of the first stop on a micropipette?

• Allows you to uptake the desired volume accurately

• Prevents the liquid from spilling out

• Increases the speed of liquid transfer

• Indicates the maximum volume capacity

When loading a sample into a well of a gel, why must you keep the plunger depressed while pulling it out?

• To ensure the sample is fully loaded into the well.

• To prevent air bubbles from forming in the sample.

• Keep the plunger depressed while removing the micropipette from the gel box so that you do not suck the liquid back up into the micropipette.

• To avoid contamination of the sample with other liquids.

What would you set the volume window to if you were pipetting 90 uL using a p-200?

• 050

• 090

• 100

• 120

Centrifuge: Purpose

• Used for the separation of fluids, gas, or liquid, based on density.

• Used for mixing substances thoroughly.

• Used for heating liquids to high temperatures.

• Used for measuring the volume of liquids.

Centrifuge: How it works

• Separation is achieved by spinning a vessel containing material at high speed.

• Separation is achieved by heating the material to high temperatures.

• Separation is achieved by filtering the material through a mesh.

• Separation is achieved by adding chemicals to the material.

What is gel electrophoresis?

• A technique used to separate DNA, RNA, or proteins based on their size and charge.

• A method for amplifying DNA sequences.

• A process for sequencing proteins.

• A technique for measuring the concentration of nucleic acids.

Image of micropipette tip above gel wells

• Illustration showing the correct position of the micropipette tip above the gel wells.

• Image of a micropipette tip submerged in gel wells.

• Diagram depicting the incorrect use of a micropipette tip.

• Photo of a gel electrophoresis setup without a micropipette.

How to load a sample into a gel using a micropipette?

• Position the micropipette tip above the well in the gel, but under the buffer solution.

• Insert the micropipette tip directly into the gel well.

• Place the micropipette tip above the gel surface without touching it.

• Submerge the micropipette tip deep into the buffer solution.