PPI and allosteric regulation

Which of the following bacterial strain was used to express RFP in our lab session?

• E. coli K-12

• E. coli DH5α

• E. coli BL21 (DE3)

• E. coli O157:H7

What makes E. coli BL21 (DE3) a widely used strain for recombinant protein expression?

• Deficiency in some proteases

• Presence of T7 RNA polymerase gene

• T7 RNA polymerase is inducible with IPTG

• All of them

PMSF stands for phenylmethanesulfonyl fluoride. What is the function is PMSF?

• It's a protease

• It's protease inhibitor

• It's a peptidase

• It's a DNAse

Which of the following best defines a cell lysate?

• A purified solution of protein extracted from a cell

• A mixture of cellular components released after breaking open cells

• An isolated group of organelles from living cells.

• A suspension of cells

What type of bond forms between nickel and his-tag?

• Ionic bond

• Covalent bond

• Hydrogen bond

• Coordinate covalent bond

What does RO water stand for?

• Refined Osmotic water

• Reverse Osmosis water

• Regular Oxygenated water

• Recycled Organic water

In which fraction, you are likely to see the protein of interest (RFP)?

• FT (Unbound)

• Wash

• Elution 1

• Elution 2

In which fraction, you are NOT likely to see the protein of interest (RFP)?

• CFE or Cell lysate

• Elution 1

• Wash

• positive contol

What is the primary function of Instant Blue in SDS-PAGE?

• To denature proteins before electrophoresis

• To separate proteins based on their charge

• To stain and visualize proteins after electrophoresis

• To break disulfide bonds in protein samples

In your poster presentation you need to finish the presentation in-

• 3 minutes

• 5 minutes

• 7 minutes

• 10 minutes