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Purpose of DNA Extraction

Back

To obtain pure DNA for analysis

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DNA Purification Methods

Back

Centrifugation, filtration, and distillation

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Purpose of DNA Purification

Back

To purify DNA after extraction

Front

Purpose of Quadrant Streaking

Back

To isolate individual colonies and then later conduct simple staining

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Purpose of Gram Staining

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To identify and differentiate Gram positive and Gram negative bacteria by color appearance

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Characteristics of Gram Positive bacteria

Back

Appear BLUE under a microscope; have a THICK peptidoglycan layer

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Characteristics of Gram Negative bacteria

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Appear PiNK under a microscope; have a THIN peptidoglycan layer

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How does Gel Electrophoresis work?

Back

It separates molecules by size and charge

Front

What is the purpose of Loading Dye in gel electrophoresis?

Back

It enhances DNA visibility during electrophoresis AND increases the density of DNA samples

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Purpose of Light Absorption/Spectroscopy of DNA Samples

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To measure the CONCENTRATION and characteristics of DNA