Biotech Unit 10 MUE

Front

Purpose of DNA Extraction

Back

To obtain pure DNA for analysis

Front

DNA Purification Methods

Back

Centrifugation, filtration, and distillation

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Purpose of DNA Purification

Back

To purify DNA after extraction

Front

Purpose of Quadrant Streaking

Back

To isolate individual colonies and then later conduct simple staining

Front

Purpose of Gram Staining

Back

To identify and differentiate Gram positive and Gram negative bacteria by color appearance

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Characteristics of Gram Positive bacteria

Back

Appear BLUE under a microscope; have a THICK peptidoglycan layer

Front

Characteristics of Gram Negative bacteria

Back

Appear PiNK under a microscope; have a THIN peptidoglycan layer

Front

How does Gel Electrophoresis work?

Back

It separates molecules by size and charge

Front

What is the purpose of Loading Dye in gel electrophoresis?

Back

It enhances DNA visibility during electrophoresis AND increases the density of DNA samples

Front

Purpose of Light Absorption/Spectroscopy of DNA Samples

Back

To measure the CONCENTRATION and characteristics of DNA