Microscope Focusing Questions

What is the first step in focusing a microscope?

• Placing the slide on the stage

• Turning on the microscope and adjusting light intensity

• Using the coarse focus knob

• Looking through the eyepiece

To what level should the light intensity be adjusted?

• 2 or 3

• 5 or 6

• 8 or 9

• 10 or 11

Why should you wiggle the stage after initially focusing?

• To clean the slide

• To adjust the light

• To ensure you're focused on the specimen, not another plane

• To center the specimen

Which objective lens is used for initial focusing?

• 4x

• 10x

• 40x

• 100x

When moving from 10x to 40x magnification, which focus knob should be used?

• Coarse focus only

• Fine focus only

• Both coarse and fine focus

• Neither, no adjustment is needed

How many complete rotations of the fine focus knob should be needed at 40x?

• Less than one

• Exactly one

• Two to three

• More than three

What must be applied before using the 100x objective?

• Water

• Saline solution

• Immersion oil

• Ethanol

What is the correct sequence for focusing at higher magnifications?

• 4x → 10x → 40x → 100x

• 10x → 4x → 100x → 40x

• 100x → 40x → 10x → 4x

• 40x → 100x → 10x → 4x

What precaution should be taken when finished using the 100x objective?

• Leave the objective in the oil

• Wipe the objective with a dry cloth

• Turn the objective out of the way before lowering the stage

• Raise the stage fully

Which of these is NOT mentioned as a step in the focusing process?

• Centering the specimen over the condenser

• Using the coarse focus to move the stage up

• Adjusting the condenser height

• Cleaning the microscope when finished