Staining Methods for Identification

What is the purpose of the endospore stain?

• To stain bacterial endospores

• To stain vegetative cells

• To differentiate between types of bacteria

What is the primary stain used in the endospore staining method?

• Malachite green

• Safranin

• Haematoxylin

What color do vegetative cells appear after endospore staining?

• Pink

• Dark green

• Colorless

What is the purpose of applying safranin in the endospore staining process?

• To counterstain decolorized cells

• To stain endospores

• To enhance the primary stain

What is the secondary stain used in the endospore staining technique?

• Crystal violet

• Safranin

• Malachite green

Which type of bacteria are typically identified using the endospore staining method?

• Gram-negative bacteria

• Gram-positive bacteria

• Both Gram-positive and Gram-negative bacteria

What is the effect of heat in the endospore staining process?

• It enhances the color of the primary stain

• It helps to penetrate the endospore

• It decolorizes the vegetative cells

What is the role of the primary stain in the endospore staining procedure?

• To stain the endospores

• To stain the background

• To stain the vegetative cells

Which of the following is a characteristic of endospores?

• They are larger than vegetative cells

• They are resistant to extreme conditions

• They are metabolically active

During the endospore staining process, what happens to the vegetative cells after decolorization?

• They retain the primary stain

• They become colorless

• They turn dark green