PCR Hindu college

The PCR technique was developed by

• Karry Mullis

• Kohler

• Milstein

• Altman

PCR is a

• DNA Sequencing technique

• DNA Amplification technique

• DNA Degradation technique

• All of these

the basic requirements of PCR reaction include

• DNA segment to be amplified

• Two oligonucleotide primers

• a heat stable DNA polymerase

• all of these

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

Denaturation involves

• heating between 40-60 C

• heating between 90-98 C

• heating between 70-72 C

• None of these

Which of the following is true regarding PCR

• Denaturation involves heating at 90-98 degree C

• Annealing involves binding of primer between 40-60 degree C

• Primer extension occurs at 72 degree C

• All of these

What does PCR stand for?

• • Phosphorylation Cycle Reaction

• • Protein Cloning Reaction

• • Polypeptide Chain Replication

• • Polymerase Chain Reaction

• Which of the following is NOT a step in PCR?

• • Denaturation

• • Annealing

• • Extension

• • Transcription

Why is a high GC content (40-60%) recommended in PCR primers?

• • It speeds up denaturation

• It ensures DNA is not amplified

• It increases primer specificity and stability

• • It prevents primer degradation