Molecular DNA (Competency 11) Review

Which sequence of DNA bases would pair with this partial strand ATG TGA CAG

• TAC TCT GGG

• TAC ACT GTC

• ATG AAA GCG

• AAC CGA TAG

What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?

• To separate DNA fragments by size

• To synthesize copies of template DNA

• To change the structure of template DNA

• To terminate the elongation of a DNA strand

During PCR, what happens during the Denaturation step?

• DNA strands are separated according to size using gel electrophoresis.

• Primers form base pairs with the single-stranded template DNA.

• DNA polymerase adds new nucleotides to the primer to elongate the new strand.

• Hydrogen bonds between bases are broken using high heat to separate DNA strands.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

• Denaturation

• Annealing

• Extension

• Synthesis

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

The enzyme that unwinds and unzips the DNA to prepare for replication is:

• helicase
• replicase
• polymerase
• synthase

In what phase of the cell cycle does DNA replication take place?

• G1
• S
• G2
• M