Quiz - Cần Thơ

How many cells in a human body?

• Around 37.2 Millions

• Around 37.2 Billions

• Around 37.2 Trillions

• Around 37.2 Quadrillion

A scientist has wanted to do a single cell experiment. The processing steps include 3 steps with the capture rate for each step as 90%. If he wants to have 10,000 cells in the final data. How many cells does he need as the input?

• 13,717 cells

• 12,345 cells

• 11,111 cells

• 90,000 cells

What is single-cell RNA-sequencing?

• A method to understand the gene mutation at the single cell level.

• A method to understand the gene expression at the single cell level.

• A method to understand the gene modification at the single cell level.

• A method to understand the gene perturbation at the single cell level.

Why is the elephant 100 times larger than human?

• Because the elephant’s cell is bigger than the human’s cell.

• Because the elephant’s cell is more complex than the human’s cell.

• Because the elephant has more cells than the human.

• Because an elephant's body requires more energy and resources to function.

What is the main advantage of single-cell RNA sequencing (scRNA-seq) over bulk RNA sequencing?

• scRNA-seq provides an average gene expression profile of a population of cells.

• scRNA-seq captures gene expression at a single-cell resolution, revealing cellular heterogeneity.

• scRNA-seq is faster and requires fewer steps than bulk RNA sequencing.

• scRNA-seq does not require bioinformatics analysis.

Which of the following is NOT a key step in single-cell RNA-seq?

• Cell isolation and dissociation

• Reverse transcription of RNA into cDNA

• Sequencing and computational analysis

• Direct measurement of protein expression

What challenge does scRNA-seq face compared to bulk RNA-seq?

• It requires fewer computational resources.

• It does not require single-cell isolation.

• The data is more sparse and prone to technical noise.

   (Dropout events are a common issue in scRNA-seq due to technical limitations.)

  
  

  
  

• It cannot identify different cell types.

Why is a unique molecular identifier (UMI) used in scRNA-seq?

• To amplify gene expression signals.

• To correct for PCR amplification bias and count individual mRNA molecules.

• To increase the number of genes detected in a single cell.

• To separate single cells from each other.

How can scRNA-seq be used to study complex biological systems?

• Identifying rare cell populations in tissues.

• Measuring the total protein content of a single cell.

• Directly sequencing DNA mutations in individual cells.

• Ignoring variability between individual cells.

What is a "dropout event" in scRNA-seq?

• A cell that is removed from the analysis due to contamination.

• A sequencing error that removes entire genes from the dataset.

• A gene that is not detected in a cell due to low mRNA capture efficiency.

   (Dropout events are a common issue in scRNA-seq due to technical limitations.)

  
  

• A gene that is only expressed in some cells but not others.