How does CRISPR work? Feng Zhang explains with a nursery rhyme

The video explains how genetic mutations can cause diseases and introduces CRISPR technology as a tool to edit the human genome. Using an analogy of proofreading a novel, the video describes how CRISPR can precisely edit DNA sequences by replacing incorrect sequences with correct ones. The process involves using CAS9 to locate and cut the DNA at the mutation site, followed by repairing the DNA with a correct sequence. The video concludes with acknowledgments.

What is the primary goal of using CRISPR technology in genetic research?

• To enhance bacterial cell functions

• To develop new types of bacteria

• To revert mutations to their normal sequences

• To create new genetic mutations

In the analogy of proofreading a novel, what does the 'typo' represent in the context of CRISPR?

• A punctuation error

• A genetic mutation

• A new sentence

• A missing page

Which of the following is NOT a method described for fixing the sentence 'twinkle twinkle big star'?

• Changing 'twinkle' to 'sparkle'

• Removing the word 'big'

• Adding the word 'little' after 'big'

• Replacing 'big' with 'little'

What role does CAS9 play in the CRISPR process?

• It repairs the DNA break

• It encodes the RNA sequence

• It identifies and cuts the mutation

• It creates new genetic sequences

How does the cell repair the DNA break after CAS9 makes a cut?

• By ignoring the break

• By duplicating the existing sequence

• By using a random DNA sequence

• By using a repair template provided