Restriction Enzymes and Gel Electrophoresis

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit

• DNA is positively charged to attracted to the negative end of the unit

• the agarose gel in negatively charged

• the agarose gel is positively charged

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

What is the purpose of gel electrophoresis?

• helps cut DNA

• count the genes in DNA

• separates DNA based on size

• allows for an exact replicated organism

Who discovered Restriction Enzymes?

• Watson and Crick

• Arber ,Natha, Linn

• Jacob and Monod

• Beadle and Tatum

Label the the bands correctly.

d

e

f

c

b

a

B2

M

D

G

J

B1

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

Lane 3

• EcoRI

• BamHI

• HaeIII

In order to insert a human gene into plasmid, both must 

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

The DNA base sequences recognized by Endonuclease are called

• Restriction Site

• Recognition Site

• Fragmentation Site

• cutting Site

The DNA base sequences recognized by Endonuclease are called

• Restriction Site

• Recognition Site

• Fragmentation Site

• cutting Site