Genomics

What are the two most important factors in Genomic research?

• patient confidentiality

• data confidentiality

• scientific availability

• scientific exploration

• financial reparations

Guess the acronym: ____________ (IGG) is a new investigative tool that combines genetic analysis with the investigation of publicly available genealogy information, which increases the pool of potential leads for law enforcement.

True or False: Direct to Consumer Genetic Testing (ie. ancestry.com) is tightly regulated

• True

• False

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.

• Deoxyribonucleotides are used in both PCR and Cycle Sequencing.

• Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

• You cannot add new nucleotides to a deoxyribonucleotide.

• Deoxyribonucleotides are NOT found in natural living cells.

• Dideoxyribonucleotides are labeled with fluorescent tags.

Tags

NGSS.HS-LS1-1

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

Place the steps of DNA Sequencing below into their correct order.

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A

Automated DNA sequencing is an improvement of Sanger's method where

• ddNTPs are used for chain termination

• PCR is used for making sequencing

• Fluorescently labelled dNTPs are used for chain termination

• Fluorescently labelled ddNTPs are used for chain termination

Which of the following is a feature of the Illumina sequencing method?

• It generates long reads

• It is a high-throughput sequencing method

• It is a single-molecule sequencing method

• It has a high error rate

Which of the following is chemical nucleotide sequencing method

• Sanger method

• Maxam Gilbert method

• Edmans method

• Automated sequencing method