Viral Plaque Assay

During the plaque assay procedure, what is poured over a hard agar surface?

• A mixture of virus, bacteria, and water

• A mixture of virus, bacteria, and COLOROTP™ soft agar

• A mixture of virus, bacteria, and PBS

• A mixture of virus, bacteria, and saline solution

What is the main objective of performing a viral plaque assay in Module I?

• To determine the pH level of the sample

• To measure the size of bacteria

• To quantify the amount of virus in a sample

• To test the effectiveness of antibiotics

What is the range of plaque counts suitable for calculating viral load in the experiment?

• 1 to 10 plaques

• 10 to 100 plaques

• 100 to 1000 plaques

• 0 to 50 plaques

Why is it important to keep the labeled agar plates warm after preparing them?

• To prevent the agar from solidifying

• To enhance the growth of microorganisms

• Because the assay only works when the base layer is warm

• To make the labels easier to read

What is the final step in the Module 1 plaque assay procedure as outlined in the document?

• Label the tape with your group initials.

• Stack the hardened plates on top of one another.

• Place the plates in an inverted position and incubate overnight at 37°C.

• Roll the agar tube between your palms for 2-3 seconds.

During the plaque assay procedure, what is poured over a hard agar surface?

• A mixture of virus, bacteria, and water

• A mixture of virus, bacteria, and COLOROTP™ soft agar

• A mixture of virus, bacteria, and PBS

• A mixture of virus, bacteria, and saline solution

What is the main objective of performing a viral plaque assay in Module I?

• To determine the pH level of the sample

• To measure the size of bacteria

• To quantify the amount of virus in a sample

• To test the effectiveness of antibiotics

What is the range of plaque counts suitable for calculating viral load in the experiment?

• 1 to 10 plaques

• 10 to 100 plaques

• 100 to 1000 plaques

• 0 to 50 plaques

Why is it important to keep the labeled agar plates warm after preparing them?

• To prevent the agar from solidifying

• To enhance the growth of microorganisms

• Because the assay only works when the base layer is warm

• To make the labels easier to read

What is the final step in the Module 1 plaque assay procedure as outlined in the document?

• Label the tape with your group initials.

• Stack the hardened plates on top of one another.

• Place the plates in an inverted position and incubate overnight at 37°C.

• Roll the agar tube between your palms for 2-3 seconds.