Thin Layer Chromatography

The substance in the mixture that has the greatest affinity for the stationary phase will travel

• fastest

• slowest

The retention factor is used

• to zero the chromatography scale

• to identify unknown compounds in a sample

• to retain solvent

• as a starting tool for chromatography

In order to compare to chromatograms what must be kept the same?

• stationary  phase

• mobile phase

• temperature

• all of the above

Which of the following factors would not affect an R_f value

• solvent used

• time taken

• temperature

• paper used

What is the primary purpose of using Thin Layer Chromatography (TLC)?

• To separate non-volatile mixtures

• To measure the pH of a solution

• To determine the boiling point of a liquid

• To calculate the density of a solid

In Thin Layer Chromatography, what is the stationary phase typically made of?

• Silica gel or alumina

• Water

• Ethanol

• Acetone

Which factor can affect the R_f value in Thin Layer Chromatography?

• Type of solvent used

• Amount of sample applied

• Color of the sample

• Shape of the TLC plate

What does the R_f value represent in Thin Layer Chromatography?

• Ratio of the distance traveled by the compound to the distance traveled by the solvent

• Rate of flow of the solvent

• Resistance factor of the compound

• Retention frequency of the compound

Why is it important to cover the TLC chamber during the development process?

• To prevent evaporation of the solvent

• To increase the temperature

• To allow more light to enter

• To keep the chamber clean

Which of the following is a common application of Thin Layer Chromatography?

• Identifying compounds in a mixture

• Measuring the viscosity of a liquid

• Determining the melting point of a solid

• Calculating the molecular weight of a compound