Serial Dilution

If the stock solution had a concentration of 25ug/uL, what is the FINAL dilution for tube C in ug/uL?

Mathematical Equivalence

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What is a serial dilution?

• A stepwise dilution of a substance in solution

• A method to prepare samples one by one

• A process of reducing the volume of a solution

• A technique to increase the concentration of a solution

How is the dilution in each tube expressed?

• As a fraction (1/X)

• As a decimal

• As a percentage

• As a whole number

How is the final sample dilution calculated?

• By multiplying the fractions from each tube

• By adding the fractions from each tube

• By subtracting the fractions from each tube

• By dividing the fractions from each tube

If Tube 1 has a 1/20 dilution and a concentration of 50 ng/ml, what is the concentration in Tube 2?

• 2.5 ng/ml

• 10 ng/ml

• 25 ng/ml

• 100 ng/ml

If the final concentration in the first tube is 200 ng sample/ml, what is the concentration in the other three tubes?

• 10 ng/ml, 0.5 ng/ml, 0.025 ng/ml

• 100 ng/ml, 5 ng/ml, 0.25 ng/ml

• 50 ng/ml, 2.5 ng/ml, 0.125 ng/ml

• 25 ng/ml, 1.25 ng/ml, 0.0625 ng/ml

Define serial dilutions in microbiology.

What is the primary purpose of performing a serial dilution in a laboratory setting?

• To increase the concentration of a solution

• To accurately measure the concentration of a solution

• To create a range of concentrations for analysis

• To purify a chemical compound

In a serial dilution, if the initial concentration is 100 mg/L and each dilution step is a 1/10 dilution, what is the concentration after three dilution steps?

• 1 mg/L

• 10 mg/L

• 0.1 mg/L

• 0.01 mg/L

Which of the following best describes a serial dilution?

• A single-step dilution process

• A multi-step dilution process where each step is a fixed dilution factor

• A process to concentrate a solution

• A method to separate components of a mixture