Plasmid

Scientists have taken advantage of __________ to use them as tools to clone, transfer, and manipulate genes by inserting genes from other species to create new genetic combinations.

• DNA fingerprints

• plasmids

• GMOs

• genomes

Tags

SB2

Circular shaped DNA found in bacteria (a)  

• plasmid

• DNA fingerprinting

• biotechnology

• vector

• GMO

• nucleus

Tags

SB2

All plasmids have a promoter region which is necessary for

• DNA to be duplicated

• DNA to be transcribed into RNA

• DNA to be inserted easily

• DNA to be cut open

What occurs during transformation?

• The use of viruses to transform or genetically

  engineer cells.

• One bacterium passes a plasmid to another through a pilus

• The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

• Genetic engineering or transformation of mammalian cells.

Observe the results above. What gene is present in the engineered plasmid?

• kanamycin resistant gene

• ampicillin resistant gene

• tetracycline resistant gene

• No plasmid is necessary to grow on plate 3.

Examine the results for group 4. Why would this group see growth without RFP in the third plate?

• The bacteria cells did not transform.

• The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.

• The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.

• Group 4 did not observe growth in the third plate.

Tags

NGSS.HS-LS3-2

Which one of these agar plates would there be no bacteria growth?

• +pGLO LB/amp

• +pGLO LB/amp/ara

• -pGLO LB/amp

• -pGLO LB

Ampicillin (antibiotic) is used in the plates to

• isolate and select for only the bacteria that have been successfully transformed

• give bacteria energy to grow

• act as a trigger to turn on the expression of the gene of interest

• give the bacteria a new trait (such as fluorescence or insulin production)

Why is calcium chloride used to make bacterial cells more competent during transformation?

• The cations from CaCl2 allow for the negative plasmid to get close to the negative plasma membrane

• The ice cold CaCl2 protects the cells from denaturing during heat shock

• The anions from CaCl2 allows the positive plasmid to get close to the positive plasma membrane

• Ice cold CaCl2 helps close membrane pores so that the plasmid cannot leave the cell

Which of the following is a common use of plasmids in genetic engineering?

• Gene cloning

• Protein synthesis

• Photosynthesis

• Cell respiration