DNA and Gel Electrophoresis

What lab technique separates DNA molecules by size in order to analyze DNA?

• Gel Electrophoresis

• Fingerprinting

• Cloning

• DNA Extraction

Gel electrophoresis separates fragments of DNA by:

• Size/weight

• Genes

• Base pairs

• Electromagnetic charge

In a DNA fingerprint, lighter-weight fragments can be found at the:

• Top

• Bottom

• Middle

• Front

Which suspect matches that of the crime scene DNA?

• 1

• 2

• 3

• 4

Tags

NGSS.HS-LS3-1

What is the purpose of gel electrophoresis in molecular biology?

• To increase the size of DNA fragments

• To separate DNA fragments based on their size

• To create DNA fragments

• To visualize proteins only

Why is gel electrophoresis an important technique in molecular biology?

• It allows for the replication of DNA.

• It enables the observation of cellular structures.

• It facilitates the separation and analysis of DNA fragments.

• It synthesizes new proteins.

Answer explanation

Gel electrophoresis is important in molecular biology as it facilitates the separation and analysis of DNA fragments.

The speed at which DNA moves through a gel is diretly related to its size.

• True

• False

Imagine that you have a sample of DNA that contains a single, specific DNA sequence. Before you run your gel, you split your sample into two tubes. You run the DNA from the first tube and use an enzyme called a restriction enzyme that cuts the DNA into two pieces at a specific point in the sequence. You then run this sample that was cut on the gel. Which if the next three lanes could be the same sample from lane two after it was cut into two pieces?

• 3

• 4

• 5

Label the the bands correctly.

d

e

f

c

b

a

M

B1

J

B2

G

D

Tags

NGSS.HS-LS3-1

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are