PCR and Gel Electrophoresis

If a restriction enzyme makes one cut in the gene, how many bands will appear on the gel?

• 0

• 1

• 2

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?

• To separate DNA fragments by size

• To synthesize copies of template DNA

• To change the structure of template DNA

• To terminate the elongation of a DNA strand

During PCR, what happens during the Denaturation step?

• DNA strands are separated according to size using gel electrophoresis.

• Primers form base pairs with the single-stranded template DNA.

• DNA polymerase adds new nucleotides to the primer to elongate the new strand.

• Hydrogen bonds between bases are broken using high heat to separate DNA strands.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

• Denaturation

• Annealing

• Extension

• Synthesis

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

Place the steps of DNA Sequencing below into their correct order.

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A

Which genetic engineering technique separates DNA fragments based on their size?

• PCR

• Gel Electrophoresis

• Gene Cloning

• CRISPR

Which genetic engineering technique amplifies the number of DNA pieces in a lab setting?

• PCR

• Gel Electrophoresis

• Gene Therapy

• CRISPR

Which DNA tech separates fragments by size/charge?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation