Recombinant DNA

Imagine you are using a new vector system for the first time. What preliminary tests would you conduct to ensure its effectiveness for your cloning project?

• Conduct transformation efficiency tests, evaluate vector stability, test gene expression, and check for mutations.

• Analyze plasmid color change

• Measure vector size only

• Assess bacterial growth rate

Tags

NGSS.HS-ETS1-3

What are some plant applications of recombinant DNA technology?

• Resist disease

• Increase nitrogen fixation

• Contain minerals, vitamins – more nutritious

• Better taste – prevent from rotting before purchase

• Have pesticides – resist insects

What are some animal applications of recombinant DNA technology?

• Leaner meat

• Fish that grow quicker

• More vitamins in food

• More nutritious milk

• Greater milk production

What are some medical applications of recombinant DNA technology?

• Produce human medications or supplements in milk of organisms

• Human insulin produced by E.coli

• Human growth hormone

• Dissolve blood clots

• Human clotting factor

Tags

NGSS.HS-LS1-1

What role do restriction enzymes play in recombinant DNA technology?

What is transformation in the context of recombinant DNA technology?

What are some industry applications of recombinant DNA technology?

• Trees that produce plastics

• Biodegradable plastic wrap, paper products from wheat

• Blue cotton – doesn’t need dyed

• Silk in goat’s milk

• Cotton that doesn’t twist – doesn’t need ironing

Tags

NGSS.HS-LS1-1

What is the purpose of heat shock treatment in plasmid insertion?

It consists of molecules of DNA joined together from different species and is inserted into a host organism.

• rDNA

• Phenotype

• Genome

• GMOs

What is recombinant DNA?

• DNA containing genes foreign to that organism

• DNA which has been impacted by epigenetics

• DNA that has restriction enzymes

• DNA that has been sequenced