Restriction Enzymes

Which RE's from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

How do we name Restriction Enzymes ?

• First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

• First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

If a restriction enzyme cuts linear DNA 3 times, how many DNA fragments were made?

• 1

• 2

• 3

• 4

If a restriction enzyme cuts a plasmid 3 times, how many DNA fragments are made?

• 1

• 2

• 3

• 4

Restriction Enzymes were primarily used as :

• Defense by Protozoa against antibiotics

• Tool by Virus to cleave Bacterial genome

• Defense by Bacteria against bacteriophages viruses

• All of the above

Restriction enzymes belong to class of enzymes:

• a).Peptidase

• b).Amylases

• c).Lipases

• d).Nucleases

The sequence that that restriction Enzymes recognizes contains how many bases?

• a).1

• b). 3

• c).6

• d).12

How do we name restriction Enzymes ?

• First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

• First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

The recognition sites for restriction enzymes are :

• a).Tautomer

• b). Pallindrome

• c).Metamere

• d).Acronym

What is the primary function of restriction enzymes in bacteria?

• To synthesize proteins

• To replicate DNA

• To cleave foreign DNA

• To repair DNA