PBS Aseptic Technique

When streaking a plate, the lid should be

• placed on the bench top, top side up 
• placed on the bench top, inside surface up
• should be held in the same hand as the plate 
• should be held in the opposite hand from the plate

When streaking a plate, the loop should be flamed

• At the beginning and end 
•  At the beginning and end and between the first 2 zones 
• At the beginning and end and between the second and third zone 
• At the beginning and end and between each zone 

Why is it important that the Petri plates are incubated upside down?

• To get dense colonies

• To get mat culture

• To get colonies with reduced growth

• To prevent moisture from condensing onto the agar surface

Flaming the neck of the test tube after uncapping the tube and before recapping is essential during culture transfer.

• True

• False

What is the agar used for?

• Nutrients for bacteria

• Sterilising equipment

• Collecting/spreading bacteria

• Stopping contamination

What is the inoculation loop used for?

• Nutrients for bacteria

• Sterilising equipment

• Collecting/spreading bacteria

• Stopping contamination

What is the bunsen burner used for?

• Nutrients for bacteria

• Sterilising equipment

• Collecting/spreading bacteria

• Stopping contamination

What is a pathogen?

• A doctor who specializes in curing diseases
• Microbes that are beneficial when present
• A special machine that is used to grow microbes
• A small living organism that can cause disease

What type of environment is best for the growth of most microorganisms?

• Hot and dry
• Sunny, cold and dry
• Warm, dark and moist
• Cold and wet

Tags

NGSS.MS-LS1-5

If you see someone in a laboratory using aseptic technique, what might that person be doing?

• Talking to a co-worker
• Looking through a microscope
• Properly cleaning equipment
• Adjusting the room temperature