Gene Expression and Cloning

Plasmids are found in bacteria. Scientists use plasmids to 

• Insert eukaryotic  genes into bacteria
• insert eukaryotic genes into humans
• insert clones into the parent organism

T6- Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day? 

• using recombinant DNA to produce human hormones from bacteria
•  testing parents for genetic disorders before they have children
• engineering fruits and vegetables that resist insects and other pests
• C. developing ways to identify criminals through DNA fingerprinting

T6- Combining DNA from 2 different organisms is called

• Bioengineering
• Biotechnology
• Recombinant DNA
• Genetic Engineering

T6- How do scientists cut DNA into smaller strands?

• restriction enzymes
• scissors
• ligase
• agarose

T6- What type of macromolecule are restriction enzymes?

• nucleic acid
• carbohydrate
• protein
• lipid

Tags

NGSS.HS-LS1-1

T6- In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

Which enzyme "glues" DNA fragments together?

• restriction enzymes
• DNA ligase
• DNA polymerase
• DNA helicase

Where is DNA located in a bacterial cell?

• nucleus
• ribosomes
• cell membrane
• cytoplasm

The first step to genetic engineering is:

• isolate gene of interest
• Cut DNA strand
• Reconnect DNA fragements
• Produce proteins

The process of putting recombinant DNA into an organism is called....

• Transformation
• Ligating
• Electrophoresis
• Incubating