Biotechnology 4.4 HW

What is the primary use of gel electrophoresis?

• To measure the pH of a solution

• To separate charged molecules such as DNA, RNA, and proteins

• To synthesize new proteins

• To measure the temperature of a solution

What is agarose, and what is its role in gel electrophoresis?

• A protein used to stain DNA

• A carbohydrate derived from seaweed used as a medium for horizontal gel electrophoresis

• A lipid used to dissolve DNA

• A type of enzyme used to cut DNA

What happens to negatively charged molecules during gel electrophoresis?

• They move toward the negative end of the gel

• They move toward the positive end of the gel

• They remain stationary

• They move toward the sides of the gel

Tags

NGSS.HS-PS2-5

NGSS.HS-PS3-5

What is the purpose of the electric field in gel electrophoresis?

• To heat the gel

• To create a magnetic field

• To move charged molecules into the gel from the wells

• To cool the gel

Tags

NGSS.HS-PS2-5

Why are RNA molecules generally smaller than DNA molecules?

• RNA molecules have fewer phosphate groups

• RNA molecules have a different sugar backbone

• RNA molecules are single-stranded

• RNA molecules are more negatively charged

What is the purpose of loading dye in gel electrophoresis?

• To make the sample heavier

• To make the sample visible

• To make the sample float

• To make the sample dissolve

What is the behavior of DNA molecules during gel electrophoresis?

• Move to the negative pole; smaller molecules move faster

• Move to the positive pole; smaller molecules move faster

• Do not have net movement to either pole

• Move to the positive pole; larger molecules move faster

Which type of gel is used to separate smaller molecules, such as proteins and very small pieces of DNA or RNA?

• Agarose gel

• Polyacrylamide gel

• Starch gel

• Cellulose gel

What is the charge of RNA molecules during gel electrophoresis?

• Positive

• Negative

• Neutral

• Variable

Which molecules do not have net movement to either pole during gel electrophoresis?

• DNA

• RNA

• Proteins

• Carbohydrates