Amgen Bacterial Transformation

What is competency for a cell?

• The insertion of a formed plasmid into the cell.

• The chemical process of switching the gene.

• Super coiled molecules formed in the plasmid of the cell.

• Change in the structure and permeability of the cell membrane.

Tags

NGSS.HS-LS1-4

How might a plasmid contribute to a selective advantage for a bacterial cell?

• Make the cell competent.

• Could provide a selective advantage for that cell.

• Make the cell wall stronger.

• Does not contribute

Tags

NGSS.HS-LS4-2

NGSS.HS-LS4-4

What is transformation?

• The use of viruses to transform or genetically

  
  

  engineer cells.

• The measure of how well cells are transformed in a new phenotype.

• The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

• Genetic engineering or transformation of mammalian cells.

Cells are made competent by;

• The incubation of the bacteria at 95C

• A process that uses calcium chloride.

• A process that uses GFP

• A process that uses polymerases

Heat shock

• Trap the plasmids inside the cell.

• Enlarges the pores of the cell.

• Provides for the death of the cell.

• Increases the disintegration of the cell wall.

The exogenous DNA used in bacterial transformation can be,

• mRNA molecule

• rDNA

• engineered plasmid

• red fluorescent protein

LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;

• Grow on the agar plate

• Will not grow on the agar plate.

• will only grow after the plate has been incubated at 45C

• will only grow after the plate has been placed in a warm water bath for 2 minutes.

Which of the following statement is true concerning transformation;

• Bacterial transformation involves the transfer of genetic material that results in the acquisition of a new trait that is stable but not inheritable.

• There are no bacterial species that go thru the transformation process naturally.

• Transformation efficiencies are a way to determine how many cells were transformed per microgram of plasmid DNA used.

• The promoter is the region at the end of the gene .

Observe the results above. What gene is present in the engineered plasmid?

• kanamycin resistant gene

• ampicillin resistant gene

• tetracycline resistant gene

• No plasmid is necessary to grow on plate 3.

Examine the results for group 4. Why would this group see growth without RFP in the third plate?

• The bacteria cells did not transform.

• The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.

• The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.

• Group 4 did not observe growth in the third plate.