College Biology DNA Technology and Genomics

Separation of DNA using electric current is called.....?

• Gel Electrophoresis
• Chromosome painting
• polymerage chain reaction
• Restrictive Enzyme Sorting 

What is the enzymatic function of restriction enzymes?

• To add new nucleotides to the growing strand of DNA.
• To join nucleotides during replication.
• To join nucleotides during transcription.
• To cleave nucleic acids at specific sites.

The unpaired nucleotides produced by the action of restriction enzymes are referred to as

• sticky end.
• base sequence.
• single strands.
• restriction fragments.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

What enzyme forms covalent bonds between restriction fragments?

• DNA primase
• DNA helicase
• DNA ligase
• DNA polymerase

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA

• I, II, IV, III, V
• III, II, IV, V, I
• II, III, V, IV, I
• III, IV, V, I, II

Organisms that have DNA from a different species artificially inserted into their cells are called

• bioremediated
• transgenic
• cultured
• a clone

GMO stands for

• Genetically Made Organism
• Growing More Organisms
• Genetically Modified Organism
• Growing Many Organisms

All of the following are advantages of GMO's except

• pest resistant crops
• more nutritional foods
• unknown long term health effects
• creating more human insulin

You have benefited from selective breeding by having more agricultural produce than would have been possible otherwise.

• True
• False