Pglo Transformation

What is the circular piece of DNA found in bacteria called?

• homologous chromosome
• sister chromatid
• plasmid
• arabinose

Making changes in the DNA of an organism is

• selective breeding
• artificial selection
• genetic engineering
• genetic transformation

Which gene WAS NOT included in the +pGlo plasmid?

• antibiotic resistance gene
• green fluorescent protein gene
• hemophilia gene

What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?

• LB

• AMP

• Ara

• +pGlo

What is the name of the plasmid we used TO TRANSORM THE BACTERIA?

• GFP

• bla

• araC

• pGLO

Which gene is responsible for making the bacteria resistant to ampicillin?

• bla
• pGLO
• araC
• GFP

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What did we use to create temperature differential to sweep plasmid inside

• heat shock
• ice
• arabinose
• CaCl₂

Which plate had bacterial growth and bacterial GLOWth?

• LB
• LB/Amp
• LB/Amp/Ara
• All

What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?

• ampicillan
• arabinose
• LB
• GFP