Microscope, Staining and Wet Mounts

Which activity might lead to damage of a microscope and specimen?

• cleaning the ocular and objectives with lens paper

• focusing with low power first before moving the high power into position

• using the coarse adjustment to focus the specimen under high power

• adjusting the diaphragm to obtain more light under high powe

While viewing a specimen under high power of a compound light microscope, a student noticed that the specimen was out of focus. Which part of the microscope should the student turn to obtain a clearer image under high power?

• eyepiece

• coarse adjustment

• fine adjustment

• nosepiece

The diagram below shows how a coverslip should be lowered onto some single-celled organisms during the preparation of a wet mount.

Why is this a preferred procedure?

• The coverslip will prevent the slide from breaking.

• The organisms will be more evenly distributed.

• The possibility of breaking the coverslip is reduced.

• The possibility of trapping air bubbles is reduced.

After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear

• larger and brighter

• smaller and brighter

• larger and darker

• smaller and darker

The table below shows the position of slides of the letter "e" on the stages of four microscopes. The image of the "e" as seen using each microscope is also shown.

Which letters correctly identify the microscopes most likely used to provide the information in the table?

• A and D –– compound light microscopes; B and C –– dissecting microscopes

• B and C –– compound light microscopes; A and D –– dissecting microscopes

• C and D –– compound light microscopes; A and B –– dissecting microscopes

• B and D –– compound light microscopes; A and C –– dissecting microscopes

A cell in the field of view of a compound light microscope is shown in the diagram below.

In which direction should the slide be moved to center this cell in the microscopic field?

• to the right and up

• to the right and down

• to the left and up

• to the left and down

Which structure in a stained cheek cell would most likely be visible when viewed through the high-power objective of a compound light microscope?

• cell wall

• ribosome

• chloroplast

• nucleus

Tags

NGSS.MS-LS1-2

The diagram below represents a hydra as viewed with a compound light microscope.If the hydra moves toward the right of the slide preparation, which diagram best represents what will be observed through the microscope?

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A specimen was viewed under the high-power objective of a compound light microscope. Its length was estimated to be 0.75 millimeter. What is the approximate length of the specimen in micrometers?

• 0.00075

• 0.75

• 75

• 750

A student using a compound microscope measured the diameter of several red blood cells and found that the average cell length was 0.008 millimeter. What is the average length of a single red blood cell in micrometers?

• 0.8

• 8

• 80

• 800