DNA Cloning and Recombinant DNA

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.
• Plasmids contain a promoter sequence that defines where transcription begins.
• Plasmids can contain an antibiotic resistance gene.
• All of the statements are true about plasmids.

In bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.

• True
• False

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• amplification

• insertion

• transformation

• screening

Which enzyme helps fuse the foreign into the new DNA?

• DNA Polymerase

• DNA ligase

The cell that able to receive recombinant DNA for cloning purpose known as

• vector

• target DNA

• plasmid

• host cell

To produce desired protein _____________ should be taken from any source like human, animal and plant.

• recombinant DNA

• Target DNA

• plasmid

• ligase

Bacteria use restriction enzymes to protect themselves from:

• other bacteria

• plants

• antibiotics

• viruses

Which of the following enzymes can cut nucleic acids?

• DNA polymerase

• restriction endonuclease

• modifying enzymes

• reverse transcriptase

Which restriction enzyme listed below was isolated from E. coli bacteria?

• EcoRI

• HindIII

• HaeII

• SmaI

Which of the following is NOT a tool used in genetic engineering?

• plasmid

• restriction enzyme

• DNA helicase

• DNA ligase