Chapter 9: Manipulating DNA

The complete set of genetic material is called:

• Genetics

• Genome

• Karyotype

• Gene

In order to cut DNA into smaller pieces we must use:

• Lipase enzyme

• Lactase enzyme

• Restriction enzyme

• Pepsin enzyme

The following enzyme left what kind of end?

• Sticky end

• Blunt end

• Straight end

• staggered end

In order to read the DNA, what do scientists need to do?

• They need to extract it and read it

• They need to cut, extract and separate it.

• They need to copy, separate and cut it.

• They need to cut, separate and copy it.

What is the name of this process?

• Pidgree

• Gel electrophoresis

• Shotgun sequencing

• Restriction enzymes

What is gel electrophoresis?

• Is used to separate and analyze the differently sized fragments

• Is used to break larger molecules into smaller

• Is used to assemble the base pairs by adding a chemical dye

• Is used to read the base sequences

What is the name of the enzyme that copies the DNA and adds nucleotides?

• Ligase

• Helicase

• RNA polymerase

• DNA polymerase

After scientists cut and separate DNA fragments, they add a ___________ to the bases so that they will be color coded.

• nucleotides

• DNA polymerase

• chemical dye

• enzymes

A computer technique that assembles all of the short fragments of DNA together to produce a full length map

• Shotgun sequencing

• Chemical sequencing

• Gel electrophoresis

• Restriction enzymes

How many genes do humans have?

• 17,000 genes

• 30,000 genes

• 15,000 genes

• 20,000 genes

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