BIO615 CH10 Genomic Libraries

What is a genomic library?

• A collection of all the genes from a species genome

• A library containing only coding genes

• A database of RNA sequences

• A collection of proteins from a species

Two basic types of DNA libraries can be created ?

• Genomic libraries

• cDNA libraries

• mRNA libraries

Steps in creating genomic library

• Screening for positive clones

• Genomic DNA extraction

• Partial digestion with a frequently used 4 base cutter

• Transformation into appropriate vectors

• Size fractionation by gel electrophoresis

Construction of genomic library using bacteriophage lambda vector

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• Introduction to a suitable host like bacterium ; Multiplication, screening, identification and characterization of clones

• Vector (plasmid) with a DNA fragment

Which enzyme is used to create cDNA from mRNA?

• Restriction enzyme

• Ribonuclease H

• Reverse transcriptase

• DNA polymerase

What type of DNA does a cDNA library represent?

• All DNA in the genome

• Only exons from mRNA

• Introns and exons

• Only non-coding DNA

What is the first step in creating a genomic library?

• Transformation into vectors

• Size fractionation by gel electrophoresis

• Genomic DNA extraction

• Screening for positive clones

What is the purpose of using probes in genomic libraries?

• To amplify DNA

• To sequence the entire genome

• To identify specific clones

• To extract genomic DNA

What type of vector is commonly used for cloning large DNA fragments?

• Yeast artificial chromosomes (YAC)

• Viral vectors

• Bacterial artificial chromosomes (BAC)

• Plasmid vectors

What is the purpose of selection of clones via plaque hybridization ?

• a method used to identify specific DNA sequences in a genomic library.

• a method used to identify any RNA sequences in genomic library.