What does chromatographic peaks represent?

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University
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Easy
Glenn Mabilangan
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15 questions
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1.
MULTIPLE CHOICE QUESTION
45 sec • 1 pt
It represents the volume of the mobile phase required to elute a compound from an LC column.
It represents the visual representation of separated compounds and the result of the separation process.
It represents the associated time for a specific analyte to travel through the chromatography system.
It represents the measure of how well two compounds are separated from each other during the chromatographic process.
Answer explanation
Each chromatographic peak represents the compound that was detected by the chromatography system. The measure of how well two compound are separated is called the resolution peak.
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following best describes the resolution of the peak?
The peaks indicate a good resolution due to column efficiency.
The peaks present a low resolution due to overlapping of peaks.
The peaks indicate a good resolution due to column selectivity
The peaks present a low resolution due to narrow and sharp peaks.
Answer explanation
A peak that produces narrow and sharp peaks indicates a good resolution due to column efficiency. Column selectivity explains that if the peaks are not overlapping with each other, it presents a good resolution.
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Size-exclusion chromatography is a technique that separates molecules based on their size using porous beads in a column. Which of the following food components would elute faster?
Gliadin
Casein
Starch
Glutenin
Answer explanation
Glutenin is a large protein, much larger in molecular weight compared to gliadin, casein, or starch. Due to its size, glutenin cannot fit into the pores of the stationary phase and bypasses them entirely, moving through the column more quickly.
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which chromatography technique is best suited for extracting essential oils?
Supercritical Fluid Chromatography
Gas Chromatography
Liquid Chromatography
Column Chromatography
Answer explanation
Essential oils are primarily composed of volatile, nonpolar compounds such as terpenes and terpenoids, making SFC an ideal technique for their extraction and analysis.
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In Gas-Liquid (G-L) Chromatography, which gas is usually used as the mobile phase?
Helium
Oxygen
Butane
Ethanol
Answer explanation
In gas chromatography, the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen.
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In affinity chromatography, what do you call the special linker attached to the stationary phase that binds to the target molecule during the process?
Ligand
Silica gel
Porous beads
Binding agent
Answer explanation
A ligand, chosen based on its specificity/selectivity and strength of interaction with the analyte, is immobilized on a suitable support material (stationary phase).
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the primary principle behind chromatography as a separation technique?
Molecules are separated based on their charge properties in the stationary phase.
Molecules interact differently with the stationary and mobile phases, leading to separation.
Separation is achieved solely by the molecular weight of the compounds.
The Stationary phase moves all molecules from the mobile phase.
Answer explanation
The stationary phase and the mobile phase interact differently with the molecules being separated. This differential interaction causes the molecules to move at different rates through the stationary phase, leading to their separation based on factors such as polarity, size, charge, or other chemical properties.
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