Trouble CT

Match the following

• rinse the slide for too long

• VVG- background is stained blue-grey and the elastic fibers do not contrast with the background.

• section was under-differentiated

• water droplet seen while looking down microscope

• did not dehydrate properly

• MT-collagen was not seen

• improper cleaned glassware

• non-specific silver staining

• No Bouin's was used

• MT- grey/unstained muscle fibers

Place the chemicals in the correct order to prepare the Verhoeff's Hx.

• Alc. Hx

• iodine

• 10% ferric chloride

Select the region that indicate the presence of muscle fibers in this Elastic VVG stain.

Identify the tissue element represented by this trichrome stain.

a

b

muscle

collagen

Acceptable or not acceptable?

• Acceptable

• not acceptable

If you use alum Hx for a Trichrome stain, you would see ​ (a)   nuclear staining.

• pale
• dark

What solution stains muscle, rbcs and cytoplasm red in the trichrome stain?

• Bouin's

• BS-AF

• alc. hx

• phosphosmolybdic/phosphotungstic acid

• aniline blue

VVG stain- acceptable/not acceptable?

• acceptable

• not acceptable

Trichrome- acceptable/not acceptable?

• acceptable

• not acceptable

What could be potential causes for not seeing reticulin fibers in the finished slide?