ELISA Quiz Part 1

What does ELISA stand for?

• Enzyme Linked Immunosorbent Assay

• Enzyme Linked Immunosuppressant Assay

• Enzyme Linked Immunoglobulin Assay

• Enzyme Linked Immunoassay

What is the purpose of the solid phase in ELISA?

• To provide a surface for antigen/antibody attachment

• To enhance the color reaction

• To dilute the enzyme

• To increase the temperature

What is adsorption in the context of ELISA?

• The process of adding an antigen/antibody to a solid phase

• The process of removing antibodies from a solution

• The process of heating the enzyme

• The process of changing the pH of the solution

What is the role of washing in ELISA?

• To separate bound from unbound reagents

• To increase the enzyme activity

• To change the color of the substrate

• To decrease the temperature

What is an antigen?

• A protein produced in response to stimuli

• A molecule that elicits antibody production

• An enzyme attached to an antibody

• A chemical that alters color

What is the role of antibodies?

• To alter color as a result of enzyme interaction

• To stop the action of an enzyme

• To produce proteins in response to antigenic stimuli

• To measure color in ELISA

What is an enzyme conjugate?

• A molecule that elicits antibody production

• A chemical that alters color

• An enzyme attached irreversibly to an antibody

• A process of stopping enzyme action

What is a chromogen?

• A protein produced in response to stimuli

• A chemical that alters color due to enzyme interaction

• An enzyme attached to an antibody

• A process of stopping enzyme action

What is the purpose of stopping in enzyme reactions?

• To produce antibodies

• To alter color

• To stop the action of an enzyme on a substrate

• To measure color in ELISA

What is the function of reading in ELISA?

• To produce antibodies

• To alter color

• To measure color spectrophotometrically

• To stop enzyme action