Discussion-2-Jan 15, 2025

Which of the following is an example of label-based quantification in proteomics?

• Spectral Counting (SpCs)

• Extracted Ion Chromatogram (XIC)

• iTRAQ

• Mascot

Which method is used in label-free quantification (LFQ) for determining peptide abundance?

• iTRAQ

• TMT

• Spectral Counting (SpCs)

• SILAC

What is the key difference between DDA and DIA in mass spectrometry?

• DDA fragments selected ions; DIA fragments all ions in predefined windows.

• DDA is label-based, while DIA is label-free.

• DIA provides less comprehensive coverage than DDA.

• DDA uses XIC, while DIA uses Mascot.

Which of the following is a chemical labeling technique used in proteomics?

• TMT

• LFQ

• Spectral Counting

• XIC

In DDA, how are ions selected for fragmentation?

• All ions are fragmented simultaneously.

• Predefined ion windows are selected.

• Top-intensity ions are prioritized for fragmentation.

• Only labeled ions are fragmented.