Recap...Chromatography

Which peak has higher k?

• A

• B

What type of this peak?

• fronting

• tailing

To obtain high column efficiency, plate height (H) should be…?

• high

• low

Which type of diffusion is independent of flow rate?

• Eddy

• Longitudinal

• Mass transfer

Band broadening due to multiple paths is…?

• Eddy

• Longitudinal

• Mass transfer

Which one should do to minimise Eddy diffusion?

• Use small packing particle size

• Loosely pack the particles

• Increase flow rate

How to reduce longitudinal diffusion?

• decrease flow velocity

• Increase flow velocity

Which of the following is correct?

• N_{column 1} = N_{column 2}

• N_{column 1} > N_{column 2}

• N_{column 1} < N_{column 2}

Which of the following is correct?

• R_S,column1 = R_S,column2

• R_S,column1 > R_S,column2

• R_S,column1 < R_S,column2

Which of the following is correct?

• α_column1 = α_column1

• α_column1 > α_column1

• α_column1 < α_column1