Tissue Sectioning Quiz

What is the primary purpose of sectioning in tissue processing?

• To stain the tissues

• To analyze the tissues

• To cut tissues into thin slices

• To preserve the tissues

Which type of section is typically cut between 4-6 microns in thickness?

• Thick Section

• Frozen Section

• Celloidin Section

• Paraffin Section

What is the purpose of trimming in the sectioning process?

• To cut the tissue into smaller pieces

• To freeze the tissue

• To expose the tissue surface

• To stain the tissue

What is the recommended angulation for the knife during sectioning?

• 0-20 degrees

• 0-15 degrees

• 0-10 degrees

• 0-5 degrees

What is the purpose of floating out sections on a water bath?

• To cool the sections

• To flatten the sections

• To dry the sections

• To stain the sections

At what temperature should mounted sections be dried in a paraffin oven?

• 5-10C below the melting point

• 10-15C above the melting point

• 2-5C above the melting point

• 0-5C below the melting point

What thickness are Celloidin sections usually cut at?

• 10-15 microns

• 4-6 microns

• 15-20 microns

• 20-25 microns

What is a common fault that occurs during tissue processing?

• Overheating

• Underheating

• Excessive staining

• Insufficient cutting

What type of microtome is typically used for cutting Celloidin sections?

• Ultramicrotome

• Cryostat microtome

• Sliding microtome

• Rotary microtome

What should be avoided to prevent tissue distortion during the floating out process?

• Insufficient stretching

• Excessive cooling

• Prolonged exposure on the water bath

• Using a dissecting needle