HLA Antibodies and Transplantation

What are donor specific antibodies?

What are the advantages of CDC in DSA detection?

• Functional assay

• Detects complement fixing antibodies

What are the limitations of CDC in DSA detection?

• Low sensitivity and specificity

• Detects both IgG and IgM

• Needs experience in interpretation

• Detects HLA and non HLA/autoantibodies

What are the advantages of DSA detection by Luminex?

• Highly sensitive

• Determine HLA specificities

• Detects IgG antibodies only

What are the limitations of DSA detection by Luminex?

• False-positive reactions

• Complement and IgM interference

• MFI values do not translate directly into the antibody level

What is the significance of DSA presence?

What is the mechanism of graft failure?

What are the methods of desensitization?

What is the incidence of HLA Ab and DSAs by SAB among AHCST candidates?