What is aseptic technique?
Bacteria Culturing and Morphology

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Biology
•
12th Grade
•
Medium
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1.
FLASHCARD QUESTION
Front
Back
Aseptic technique is a set of practices used to prevent contamination by pathogens during laboratory procedures, ensuring that all equipment and workspaces are sterile before culturing.
2.
FLASHCARD QUESTION
Front
Why is it important to sterilize equipment before culturing bacteria?
Back
Sterilizing equipment before culturing bacteria is crucial to prevent contamination, which can lead to inaccurate results and the growth of unwanted microorganisms.
3.
FLASHCARD QUESTION
Front
What does it mean if a bacterium is Gram positive?
Back
Gram positive bacteria have a thick peptidoglycan layer in their cell wall, which retains the crystal violet stain used in the Gram staining procedure, causing them to appear purple under a microscope.
4.
FLASHCARD QUESTION
Front
What does it mean if a bacterium is Gram negative?
Back
Gram negative bacteria have a thinner peptidoglycan layer and an outer membrane, which does not retain the crystal violet stain, causing them to appear pink after Gram staining.
5.
FLASHCARD QUESTION
Front
What is peptidoglycan and where is it found?
Back
Peptidoglycan is a polymer that forms a protective layer in the cell wall of bacteria, providing structural support and shape.
6.
FLASHCARD QUESTION
Front
What is the role of the cell wall in bacteria?
Back
The cell wall provides structural integrity, protects against osmotic pressure, and helps maintain the shape of the bacterium.
7.
FLASHCARD QUESTION
Front
What is the significance of hand washing in aseptic technique?
Back
Hand washing is a critical part of aseptic technique as it reduces the risk of transferring pathogens from hands to laboratory equipment or cultures.
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