chm 260 c4

What is chromatography?

• Separation of component in a mixture

• Time for compound to reach detector

• Mixing of two orbital overlapping by covalent bond by sharing their electron

• The separation of two resolution peak

how many methods of chromatography in this chapter?

• 2

• 4

• 3

• 5

what is adsoption chromatography?

• separation using porous matrix sieve

• separation for ionic compound

• separation based on charged analyte

• separation based on solubility

what is size exclusion chromatography?

• separation of polar compound

• separation based on charged compound

• separation based on size using porous matrix sieving

• none of above

what is ion exchange chromatography?

• separation based on charged analyte

• separation based on solubility

• separation based on polarity

• separation based on size exclusion

what is partition chromatography?

• separation of non polar compound

• separation based on size using sieve matrix

• separation based on solubility

• separation of polar compound

how to improve peak separation?

• increase the solubility

• improve the resolution

• increase the retention time

• increase the dead time

The Tr for A = 17.63 and Tr for B = 16.40. The length of column is 30 cm. Peak width for A and B were given as 1.11 min and 1.21 min, respectively. Calculate the column resolution.

• 1.06

• 2.23

• 2.46

• 3.11

The Tr for A = 17.63 and Tr for B = 16.40. The length of column is 30 cm. Peak width for A and B were given as 1.11 min and 1.21 min, respectively. Calculate the average number plate.

• 3443.34

• 32.34523

• 300032.22

• 3.21222

The Tr for A = 17.63 and Tr for B = 16.40. The length of column is 30 cm. Peak width for A and B were given as 1.11 min and 1.21 min, respectively. Calculate the average peak height.

• 0.00087

• 0.87

• 87

• 0.0087