This is known as Edwards Syndrome, can you identify the chromosomal mutation?

This persons chromosomes are normal

What is the objective of the Edvo-Kit #330 experiment?

To gain hands-on experience of the principles and practice of Polymerase Chain Reaction (PCR)

To understand the structure of DNA

To learn about the storage instructions for laboratory equipment

To study the effects of temperature on enzyme activity

What is the first step in the PCR cycle depicted in the diagram?

Anneal 2 primers at 40°C - 65°C

During which step of the PCR cycle are the primers annealed?

Anneal 2 primers at 40°C - 65°C

Why is PCR considered ideal for forensic analysis and biological sample determination of paternity?

Because it can amplify DNA rapidly and requires very little starting material

Because it requires a large amount of starting material

Because it is a time-consuming process

Because it does not require precise temperature control

What common problem occurs during PCR due to contamination?

The amplification of unwanted products due to nonspecific annealing of primers

The DNA sample becomes radioactive

The annealing temperature becomes too high

The PCR reaction takes longer than expected

What does Figure 2 illustrate about PCR?

The exponential increase of DNA copies followed by a plateau phase

The linear increase of DNA copies with each cycle

The constant number of DNA copies after a certain number of cycles

The decrease of DNA copies in the plateau phase

What is the objective of the PCR amplification of DNA experiment?

To gain hands-on experience of the principles and practice of Polymerase Chain Reaction (PCR)

To analyze the PCR products by agarose gel electrophoresis only

To learn about laboratory safety and equipment usage

To record observations in a laboratory notebook

What is the first step you should take before starting the experiment?

Read and understand the instructions completely

Predict the results of your experiment

What should you do after completing the experiment?

Interpret the results to support or contradict your hypothesis

Discard your hypothesis regardless of the results

Ignore the results and repeat the experiment immediately

Leave the laboratory without cleaning up

What is the first step in performing the Polymerase Chain Reaction according to the module?

Add 20 µL Primer mix, 20 µL Ultra-pure water, and 5 µL DNA template to the PCR tube.

Gently mix the PCR sample mixture.

Transfer one PCR EdvoBead™ PLUS to the labeled 0.2 mL "PCR" tube.

Centrifuge to collect the sample at the bottom of the tube.

What is the purpose of adding 15 µL Ultra-pure water to the PCR sample mixture in tube "O"?

To dilute the PCR sample mixture before electrophoresis.

To dissolve the PCR EdvoBead™ PLUS completely.

To collect the sample at the bottom of the tube.

What is the purpose of the 100 bp ladder in the experiment?

To provide a size reference for DNA fragments

To measure the volume of samples

To control the temperature of the PCR

To change the color of the samples

Which item is highly recommended for visualizing DNA after PCR in SNP analysis of the PTC gene?

UV Transilluminator or Blue Light Visualization System

What does SNP stand for in the context of genetics?

Synchronized Nucleotide Process

In the context of the PTC sensitivity gene, what does the dominant allele (T) confer?

A higher sensitivity to all tastes

A lower sensitivity to bitter tastes

What is the role of the restriction enzyme HaeIII in the experiment?

To cut the DNA at specific sites

To ligate DNA fragments together

What is the correlation that students will investigate in the final module of the experiment?

The correlation between their genotype and their ability to taste PTC

The correlation between DNA concentration and gel electrophoresis results

The correlation between the number of cycles in PCR and the amount of DNA amplified

The correlation between the length of the amplified DNA and the restriction sites

What is the objective of the experiment described in the document?

To isolate human DNA and use PCR to amplify the TAS2R38 gene, which is responsible for detecting the bitter taste of PTC

To isolate plant DNA and use PCR to amplify the TAS2R38 gene

To compare the DNA of different species using gel electrophoresis

To test the effectiveness of different types of PCR reagents

What is the final step in the PCR cycle settings provided in the document?

Final Extension: 72°C for 5 minutes

Initial denaturation: 94°C for 4 minutes

Denaturation: 94°C for 30 seconds

Annealing: 64°C for 30 seconds

What is the purpose of incubating the solution at 55°C in the DNA isolation process?

To burst (lyse) the cells and release the DNA

To pellet the cells at the bottom of a micro-centrifuge tube

To isolate the DNA in the supernatant

What is the role of centrifuging in the process of DNA isolation from cheek cells?

To separate the cell debris from the DNA

To mix the lysate with the solution

To add the lysis buffer to the solution

To incubate the solution at 99°C

What is the purpose of performing a restriction digest on the PCR product in Module III?

To determine if a single nucleotide polymorphism (SNP) is present at nucleotide 145

To label the DNA with fluorescent markers

To separate the DNA fragments by size

Mi 2.1 Test

Authored by Lyndsey Littlefield

Science

12th Grade

NGSS covered

Used 3+ times

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DRAG AND DROP QUESTION

1 min • 1 pt

Match the disorders with their correct definition
Single gene disorder - (a)
Multifactorial Disorders - (b)
Chromosomal Disorders - (c)
Mitochondrial Disorders - (d)

Caused by changes in the DNA of one gene

Caused by environmental factors and mutations

Caused by missing or extra copies of chromosomes

Caused by mutations in mitochondrial DNA

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

By looking at the chromosomes how can you tell that this person is biologically female?

Because there are two X chromosomes

Because there are more chromosomes than in a male

Tags

NGSS.HS-LS3-1

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

By looking at the chromosomes is this individual biologically male or female? How do you know?

Female, because they have two X chromosomes

Male, because they have one X and one Y chromosome

Tags

NGSS.HS-LS3-1

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the mutation in this persons chromosomes?

There is only one X

7 is shaped like a hockey stick

There's an extra 21

1 is too curved

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

This is called Turners syndrome, can you notice the mutation in the chromosomes?

Both chromosomes 1 are bent

There are too many chromosomes

There are too many of chromosome 3

There is no Y chromosome

Tags

NGSS.HS-LS3-2

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

These are the chromosomes of someone with Kleinfelters syndrome. What is different about their chromosomes?

There is only one Y chromosome

One of the 5th chromosomes is too curved

There is an extra X chromosome

There are too few chromosomes

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is a karyotype?

A karyotype is a type of DNA sequencing technique.

A karyotype is a visual representation of the chromosomes in an individual's cells.

A karyotype is a genetic disorder caused by an extra chromosome.

A karyotype is a type of microscope used to study chromosomes.

Tags

NGSS.HS-LS3-1

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Mi 2.1 Test

Match the disorders with their correct definitionSingle gene disorder - (a) Multifactorial Disorders - (b) Chromosomal Disorders - ​ (c) Mitochondrial Disorders - ​ (d)

By looking at the chromosomes how can you tell that this person is biologically female?

By looking at the chromosomes is this individual biologically male or female? How do you know?

What is the mutation in this persons chromosomes?

This is called Turners syndrome, can you notice the mutation in the chromosomes?

These are the chromosomes of someone with Kleinfelters syndrome. What is different about their chromosomes?

What is a karyotype?

This is known as Edwards Syndrome, can you identify the chromosomal mutation?

What is the objective of the Edvo-Kit #330 experiment?

How are the PCR products analyzed in the Edvo-Kit #330 experiment?

Access all questions and much more by creating a free account

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Popular Resources on Wayground

Discover more resources for Science

Match the disorders with their correct definition
Single gene disorder - (a)
Multifactorial Disorders - (b)
Chromosomal Disorders - (c)
Mitochondrial Disorders - (d)