lecture 16 part 2

In which direction does DNA synthesis/replication occur?

• 3’ to 5’

• 5’ to 3’

• 2’ to 4’

• 4’ to 2’

Where are new nucleotides added during DNA synthesis?

• 5’ end

• 3’ end

• Middle of the strand

• Both ends

What is the major DNA polymerase in E. coli?

• DNA Pol I

• DNA Pol II

• DNA Pol III

• DNA Pol IV

What is the function of the 5’ to 3’ exonuclease activity of DNA polymerase I?

• It synthesizes new DNA strands.

• It removes nucleic acids ahead of the polymerase.

• It adds nucleotides to the 3’ end of the DNA.

• It repairs mismatched base pairs.

What process is used to remove DNA ahead of the polymerase?

• Base pairing

• Nick translation

• DNA replication

• Transcription

How is DNA replication on the leading strand described?

• Discontinuous

• Fragmented

• Straight-forward

• Random

How is DNA replication on the lagging strand described?

• Discontinuous

• Fragmented

• Straight-forward

• Random

What are the small fragments called that are synthesized on the lagging strand?

• Watson fragments

• Crick fragments

• Okazaki fragments

• Franklin fragments

What is the role of primase in DNA synthesis?

• It synthesizes a long piece of DNA

• It provides the 5' OH for DNA Pol III

• It synthesizes a short piece of RNA

• It removes nucleotides from the DNA strand

What enzyme is responsible for replacing RNA primers with DNA in DNA synthesis?

• DNA polymerase I

• DNA polymerase III

• RNA polymerase

• DNA helicase