Microbial Genetics Quiz

Which of the following describes the structure of a plasmid?

• Single-stranded linear DNA

• Double-stranded circular DNA

• Single-stranded RNA

• Linear RNA

What is the role of the F-plasmid in bacteria?

• Antibiotic resistance

• Gene cloning

• Bacterial conjugation

• Production of colicins

What type of plasmid is responsible for degrading unusual compounds like toluene?

• Virulence plasmid

• Degradative plasmid

• Col-plasmid

• R-plasmid

Which of the following is NOT a characteristic of a good vector?

• Contains an origin of replication

• Very large in size

• Includes multiple cloning sites

• Has selectable markers

What does a high-copy-number plasmid imply?

• It carries multiple genes.

• It is maintained in low quantities in the host cell.

• It can replicate independently and exist in large quantities in a host cell.

• It is resistant to antibiotics.

What is the purpose of recombinant DNA technology?

• To combine DNA from different sources

• To produce new genetic combinations

• To develop genetically modified organisms

• All of the above

Which plasmid is widely used in genetic engineering and contains the lacZ gene?

• pBR322

• pUC19

• ColE1

• RP4

What is insertional inactivation used for in genetic engineering?

• To identify recombinant plasmids

• To amplify plasmid DNA

• To produce therapeutic proteins

• To degrade antibiotics

Which plasmid is associated with antibiotic resistance?

• Col-plasmid

• R-plasmid

• Virulence plasmid

• Degradative plasmid

What is a vector?

• A molecule carrying genetic material into a host cell

• A type of plasmid specific to antibiotic resistance

• A bacterial chromosome

• A gene encoding colicins