HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Which of the following are correct statement about the boxes labeled A, B, C, and D?

• The box labeled A has a function to deliver a specific composition of liquid to the column at a precise flow rate.

• The box labeled B has the function to remove dissolved gas, which can cause bubbles in the flow path of pumps and detectors.

• The box labeled C, where the sample is separated in HPLC.

• The box labeled D is a device that introduce a precise amount of a sample into the system's column.

The results of observations using HPLC are called ....

• Spectroscopy

• Spectrum

• Chromatogram

• Chromatograph

Qualitative analysis on HPLC is known based on . . . .

• mass of sample

• absorbance

• peak area

• retention time

Quantitative analysis on HPLC is known based on . . . .

• mass of sample

• absorbance

• peak area

• retention time

Give a short conclusion based on the picture!