Bioengineering - Module 3 - Genetic Engineering (PS & pGLO)

What is this structure?

• nucleotide

• ribosome

• protein

• amino acid

What makes up the rungs (steps) of a DNA ladder?

• sugars & phosphates

• nitrogen bases

• sugars

• genes & chromosomes

What makes up the SIDES of a DNA ladder?

• nitrogen bases

• sugars & phosphates

• genes & chromosomes

• RNA

The process to go from mRNA to a protein is called ____

• transcription
• rRNA
• replication
• translation

Which of the following is the complementary mRNA sequence to this DNA strand: AACTGAA

• UUGACUU

• TTGACTT

• AACTGAA

• AACUGAA

Which gene is responsible for making the bacteria resistant to ampicillin?

• bla
• pGLO
• araC
• GFP

Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?

• GFP
• araC
• restriction enzyme

• beta lactamase

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What did we use to create temperature differential to sweep plasmid inside

• heat shock
• ice
• arabinose
• CaCl₂

Which plate had bacterial growth and bacterial GLOWth?

• LB
• LB/Amp
• LB/Amp/Ara
• All